RNA Extraction and Preservation Test, Price, Normal Range | Sprint Diagnostics Hyderabad
Patient Preparing : No specific preparation is required for this procedure. However, it's crucial that the sample is collected, stored, and transported following proper guidelines to prevent RNA degradation.
RNA Extraction and Preservation represent a crucial step in many biomedical research and diagnostic procedures. Ribonucleic acid (RNA) is a type of nucleic acid present in all living cells and plays a pivotal role in protein synthesis and other vital cellular activities.
RNA extraction is the process by which RNA is separated from a biological sample. Because RNA is often the target of many research and diagnostic tests - particularly in genetic, cancer, and infectious disease studies - its extraction is an essential laboratory procedure. Once the RNA is extracted, it must be adequately preserved to prevent degradation, as RNA is highly sensitive to enzymes present in cells and the environment.
|RNA Extraction and Preservation
|No specific preparation is required for this procedure. However, it's crucial that the sample is collected, stored, and transported following proper guidelines to prevent RNA degradation.
|Price in Hyderabad
What is RNA extraction?
RNA extraction is a process used to isolate RNA from biological samples. This RNA can then be used in various laboratory procedures such as RT-PCR, Northern Blotting, and RNA sequencing.
Why is RNA preservation necessary?
RNA is highly sensitive and can degrade quickly if not properly handled and stored. Preservation ensures that the RNA remains intact and usable for downstream applications.
Home Sample Collection
How is RNA extraction carried out?
RNA extraction typically involves three steps: cell lysis (breaking open the cell), removal of DNA and proteins, and RNA recovery. There are several extraction methods, but a common one involves using chemical reagents and a centrifuge.
How is the extracted RNA preserved?
Extracted RNA is often preserved by storing it at very low temperatures, typically at -80°C. It's also essential to avoid RNase, an enzyme that degrades RNA, during the extraction and storage process.
What can affect the quality of the extracted RNA?
Several factors can affect RNA quality, including the initial sample quality, the extraction method used, handling during the extraction process, and storage conditions.
Why is RNA extraction important in research and diagnostics?
RNA extraction is crucial in many areas of research and diagnostics because it allows scientists to study gene expression, investigate genetic diseases, detect viruses, and more.
What is RNase, and how can it affect my RNA sample?
RNase is an enzyme that breaks down RNA. It's ubiquitous and can quickly degrade RNA if the sample is not properly handled and stored.
What precautions are taken during RNA extraction to prevent degradation?
During RNA extraction, samples are handled with care to avoid RNase contamination. All surfaces and tools are cleaned or treated to deactivate RNase, and in some cases, inhibitors are added to the sample to further prevent RNase activity.
What are the uses of the RNA after extraction and preservation?
Once the RNA is extracted and preserved, it can be used in several laboratory procedures, including reverse transcription-polymerase chain reaction (RT-PCR), microarray analysis, and RNA sequencing, which are used in disease diagnostics, genetic studies, and cancer research.
How long can the extracted RNA be preserved?
If properly stored at -80°C, the extracted RNA can be preserved for several years. However, it's generally recommended to use the RNA as soon as possible after extraction for the best results.
What is the role of RNA in protein synthesis?
RNA carries the genetic information from DNA to the ribosomes, where protein synthesis occurs. This process is known as transcription and translation.
Can RNA extraction be performed at home?
RNA extraction requires specialized equipment and reagents, and must be carried out under controlled conditions to prevent RNA degradation. Therefore, it is not recommended to attempt RNA extraction at home.
Can RNA extraction and preservation help in detecting viruses like COVID-19?
Yes, RNA extraction is a vital step in the RT-PCR test used to diagnose COVID-19 and many other viral infections. The virus's RNA, if present in the sample, is extracted and then amplified for detection.
How can I ensure that the RNA extracted is of good quality?
The quality of extracted RNA can be assessed using various methods like spectrophotometry and agarose gel electrophoresis. Proper sample collection, careful extraction, and appropriate storage are crucial for ensuring RNA quality.
What is the difference between DNA and RNA extraction?
While the basic principles are similar - cell lysis, removal of unwanted components, and nucleic acid recovery - there are specific differences. For instance, RNA extraction includes an additional step to remove DNA, and the extracted RNA must be handled more carefully due to its sensitivity to degradation.
What is the future of RNA extraction and preservation?
The importance of RNA extraction and preservation is expected to grow with advances in genetic and molecular biology research, disease diagnostics, and personalized medicine.
What are some of the challenges with RNA extraction and preservation?
Some of the challenges include maintaining the integrity of RNA during extraction, preventing degradation during storage, and ensuring that the extracted RNA is free of DNA and protein contamination.
Can RNA extraction provide information about gene expression?
Yes, studying RNA - particularly messenger RNA (mRNA) - allows researchers to understand which genes are being expressed in a particular cell at a specific time.
What is the role of RNA in genetic research?
In genetic research, RNA plays a crucial role in understanding how genes are expressed and regulated. This can help in studying various genetic disorders and diseases.
Are there different methods of RNA extraction?
Yes, there are several methods of RNA extraction, including manual methods using reagents and automated methods using specialized machines. The choice of method depends on the type and quantity of the sample, as well as the intended use of the RNA.
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