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Real Time PCR for Zika Virus

The Real Time PCR for Zika Virus is a test used to detect the Zika virus in a person's blood. This diagnostic tool uses the method of Real Time Polymerase Chain Reaction (PCR) that amplifies the genetic material of the virus making it detectable.

Zika virus is a mosquito-borne flavivirus closely related to the dengue, yellow fever, and West Nile viruses. It was first identified in the Zika forest of Uganda in 1947. Its mild symptoms and limited geographical spread kept it from being recognized as a significant health threat until the major outbreak in Brazil in 2015 that linked the virus to a spike in birth defects.


  • Test NameReal Time PCR for Zika Virus
  • Sample TypeBlood
  • Preparations RequiredNone
  • Report Time3 days

Though the majority of individuals infected with the Zika virus do not experience symptoms, those that do often report fever, rash, joint pain, and conjunctivitis. More severe complications can include Guillain-Barre syndrome and congenital abnormalities in infants born to infected mothers.

Now, let's delve into the Frequently Asked Questions related to this test.

Home Sample Collection Process

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Sample Collection by Phlebotomist
Sample Collection by Phlebotomist
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Reporting of the sample at lab
Reporting of the sample at lab
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Frequently Asked Questions

The test may be ordered if you have symptoms of Zika virus infection and you live in or have recently traveled to an area where Zika is known to be present.

The test requires a blood sample, which is drawn from a vein in your arm.

The test is considered quite safe, with minimal risks that might be associated with blood collection, such as light-headedness or infection at the site of the needle prick.

There are no special preparations needed for the test.

The Zika virus is primarily transmitted through the bite of an infected Aedes species mosquito. It can also be transmitted through sexual contact, from mother to fetus, and potentially through blood transfusion.

A positive result indicates that the Zika virus RNA is present in the blood sample and the person is likely infected with the virus.

Yes, infection with Zika virus during pregnancy can cause a serious birth defect called microcephaly and other severe fetal brain defects.

The best way to prevent Zika is to avoid mosquito bites in areas where the virus is present. This can be done by wearing long-sleeved shirts and long pants, staying in places with air conditioning and window and door screens, and using insect repellents.

There is currently no vaccine or specific antiviral treatments for Zika virus. Care is based on symptoms and includes rest, fluids, and use of common over-the-counter medications for fever and pain.

If you test positive for Zika virus, follow the advice of your healthcare provider. Rest, stay well-hydrated, and avoid mosquito bites for the first week of your illness to prevent the spread of the virus.

Yes, the Real Time PCR for Zika virus can detect the virus in individuals who do not show any symptoms but are infected.

Yes, the test is designed to specifically identify the Zika virus and should not cross-react with other flaviviruses like dengue or chikungunya.

The test is highly accurate but a negative result does not completely rule out the possibility of an infection. It may be necessary to perform additional tests.

The test's accuracy can be affected by the timing of the test. The virus is usually detectable in the blood within the first week after symptom onset.

If symptoms persist despite a negative test result, it is recommended to consult with a healthcare provider. Further diagnostic testing may be required.

The Real Time PCR for Zika Virus is an effective diagnostic tool that aids in the identification and management of Zika virus infection. By understanding the nature of the test and its implications, individuals can make informed healthcare decisions.

If you're living in or traveling to an area where Zika virus is prevalent, or if you've been exposed to the virus (like through sexual intercourse with a partner who has been in such areas), you should consider getting tested. However, the frequency of testing should be discussed with your healthcare provider as it depends on your personal risk factors.

Current scientific understanding suggests that once a person has been infected with Zika virus, they are likely to be protected from future infections. However, research is ongoing and it's always best to take preventative measures.

No, the Real Time PCR for Zika Virus test is not designed to detect past infections. It only detects current, active infections. If you need to determine past exposure to Zika virus, other types of tests that measure the body's immune response to the virus can be considered.

According to the World Health Organization, there have been no reports of infants getting Zika virus through breastfeeding. Mothers in areas affected by the Zika virus epidemic should continue to breastfeed their infants as per the standard recommendations.

If your test results are abnormal, you should consult an Infectious Disease Specialist or a General Practitioner who can guide you on the next steps based on your symptoms and medical history.

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