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Coombs Test - Direct with Reflex Typing (IgG, IgM, IgA, C3c, and C3d)

Coombs Test - Direct with Reflex Typing (IgG, IgM, IgA, C3c, and C3d)

The Coombs Test, more specifically, the Direct Coombs Test with Reflex Typing, is an enhanced version of the regular Direct Coombs Test. It is used to detect antibodies that are attached to the surface of red blood cells and is critical in diagnosing and managing autoimmune hemolytic anemia, hemolytic disease of the newborn, and monitoring blood transfusion reactions. In contrast to the regular Direct Coombs Test, the Reflex Typing method also identifies the specific type of antibodies or complement proteins (IgG, IgM, IgA, C3c, and C3d) that are bound to the red blood cells.

This identification is crucial as it helps doctors understand the underlying cause of the autoimmune response and tailor the treatment more effectively. For example, differentiating between the various immunoglobulin classes (IgG, IgM, IgA) and complement components (C3c and C3d) can provide insights into the cause of hemolysis and guide therapeutic decisions.


  • Test NameCoombs Test - Direct with Reflex Typing (IgG, IgM, IgA, C3c, and C3d)
  • Sample TypeBlood
  • Preparations RequiredNo fasting or specific preparation is required.
  • Report Time6 hours

What is the Coombs Test with Reflex Typing?

The Direct Coombs Test with Reflex Typing not only detects the presence of antibodies bound to the surface of red blood cells but also identifies which specific antibodies or complement proteins are involved. This information is valuable for pinpointing the cause of the condition and guiding treatment.

How is the Coombs Test - Direct with Reflex Typing performed?

A blood sample is taken and mixed with anti-human globulin. If clumping occurs, indicating the presence of antibodies, further tests are done to identify the specific type of antibodies or complement proteins involved.

Home Sample Collection Process
1
Book your convenient slot
Book your convenient slot
2
Sample Collection by Phlebotomist
Sample Collection by Phlebotomist
3
Reporting of the sample at lab
Reporting of the sample at lab
4
Download Reports
Download Reports
Frequently Asked Questions

A positive result indicates that antibodies or complement proteins are attached to the red blood cells. The reflex typing helps identify which specific antibodies or complement proteins are involved, providing insight into the cause and guiding treatment.

A negative result suggests that there are no antibodies or complement proteins attached to the red blood cells, making conditions like autoimmune hemolytic anemia less likely.

Identifying the specific antibodies or complement proteins is essential for understanding the cause of the autoimmune response. Different types of antibodies may suggest different underlying conditions or causes, and this information is crucial for tailoring the treatment plan.

Yes, this test can be used during pregnancy to detect antibodies that might affect the fetus, especially in cases of Rh incompatibility or other antibody-mediated conditions that may cause hemolytic disease of the newborn.

Each of these components plays a different role in the immune response. For example, IgG is often involved in autoimmune hemolytic anemia, while IgM is more likely to react at lower temperatures and may be associated with cold agglutinin disease. The presence of complement proteins C3c and C3d suggests complement activation, which is part of the body's immune response.

The risks are minimal and similar to those of a regular blood draw, such as slight pain, bruising, or bleeding at the site where the needle is inserted.

Yes, certain medications can interfere with the results of the test. It’s important to inform your doctor about all medications and supplements you are taking.

If the Coombs Test with Reflex Typing is positive, it is advisable to consult a hematologist for further evaluation and guidance regarding the underlying cause and appropriate management.

By identifying the specific antibodies or complement proteins involved, the Coombs Test with Reflex Typing helps doctors understand the underlying cause of the hemolysis in autoimmune hemolytic anemia and tailor the treatment accordingly.

No, this test specifically detects immune-mediated hemolytic anemia, where antibodies or complement proteins are involved. It does not detect other types of hemolytic anemia that result from different causes.

Yes, the Coombs Test with Reflex Typing can be used to monitor the levels of antibodies or complement proteins on red blood cells, helping evaluate the effectiveness of treatment.

The Coombs Test - Direct with Reflex Typing is a highly specialized test that not only detects the presence of antibodies bound to red blood cells but also identifies the specific type of antibodies or complement proteins involved. This information is invaluable in understanding the cause of the autoimmune response and in devising an effective treatment strategy. It is important for individuals undergoing this test to consult their doctor for proper interpretation and guidance.

Coombs Test - Indirect with reflex Antibody Screening (3 cell panel)
₹ 1400
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