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Blood Grouping and Typing-Tube Agglutination Method

Blood grouping and typing using the Tube Agglutination Method is a laboratory technique commonly used to determine an individual's ABO and Rh blood group. Blood grouping and typing are fundamental procedures in transfusion medicine, ensuring the compatibility of blood given during transfusions. Blood is categorized into different groups based on the presence or absence of specific antigens on the surface of red blood cells. The two most important classifications to know for blood transfusions are the ABO and Rh systems. The Tube Agglutination Method is one of the oldest and traditional methods for blood grouping and typing. This method helps in determining an individual’s ABO and Rh blood group by observing the agglutination or clumping of red blood cells.

  • Test Name Blood Grouping and Typing (ABO and Rh) - Tube Agglutination Method
  • Sample Type Blood
  • Preparations Required No specific preparation is required for this test.
  • Report Time 4 Hours

The Tube Agglutination Method involves mixing a small sample of blood with specific antisera in test tubes. The antisera contain antibodies that will cause the blood cells to agglutinate if the corresponding antigens are present on their surface. The tubes are then gently mixed and left at room temperature. Afterward, the technician observes the tubes for agglutination. The presence or absence of agglutination is used to determine the blood group. For precise and expert diagnostic tests in Hyderabad, Sprint Diagnostics provides reliable services at your nearest locations.

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Frequently Asked Questions

The Tube Agglutination Method is a traditional method for blood grouping and typing. It involves mixing a blood sample with antisera in test tubes, and observing for agglutination or clumping, which indicates the presence of specific antigens.

Blood grouping and typing is a crucial diagnostic technique used to identify an individual's blood group.

Blood grouping and typing are essential for safely conducting blood transfusions, organ transplants, and determining maternal-fetal compatibility during pregnancy. Using incompatible blood can lead to severe and potentially fatal reactions.

The Tube Agglutination Method, while widely employed for blood grouping, has limitations such as subjectivity in interpretation, sensitivity to environmental factors, and potential time constraints.

There are no significant risks associated with this test, apart from the minor risks related to the blood draw.

The turnaround time for blood grouping and typing using the Tube Agglutination Method is generally 1-2 hours.

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