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auramine-rhodamine-stain-for-screening-of-tubercle-bacilli

Auramine Rhodamine Stain for Screening of Tubercle Bacilli

Tuberculosis (TB) is a contagious and often severe airborne disease caused by infection with Mycobacterium tuberculosis (MTB) bacteria. Early detection and treatment of TB is crucial in controlling its spread and mitigating health risks. The Auramine Rhodamine Stain Test for screening of Tubercle Bacilli is a frontline diagnostic tool to aid in the identification of MTB. This comprehensive guide provides you with essential information about this test, its implications, and its role in TB management.


  • Test Name Auramine Rhodamine Stain for Screening of Tubercle Bacilli
  • Sample Type Sputum
  • Preparations Required None
  • Report Time 5 hours

The Auramine Rhodamine Stain Test is a fluorescent staining method used to detect acid-fast bacilli, including Mycobacterium tuberculosis. This test uses auramine and rhodamine dyes, which bind to the mycolic acids in the cell wall of the bacteria, rendering it fluorescent under specific light conditions. This attribute makes the detection of tubercle bacilli quicker and more efficient compared to traditional staining methods, thus enabling a faster diagnosis of tuberculosis.

The test holds significance in its ability to facilitate early detection and appropriate treatment of TB, particularly in high-risk populations. It is a highly sensitive method, capable of detecting even a small number of bacilli in the sample. Given the high prevalence of TB worldwide, especially in developing countries, the Auramine Rhodamine Stain Test plays a pivotal role in global TB control.

Home Sample Collection Process

1
Book your convenient slot
Book your convenient slot
2
Sample Collection by Phlebotomist
Sample Collection by Phlebotomist
3
Reporting of the sample at lab
Reporting of the sample at lab
4
Download Reports
Download Reports
Frequently Asked Questions

The test is primarily used to detect Mycobacterium tuberculosis in a sputum sample. It helps diagnose TB, especially in high-risk populations.

TB primarily affects the lungs, leading to symptoms like persistent cough, chest pain, and difficulty in breathing. Other symptoms include weight loss, fever, Night Sweats, and fatigue.

A sputum sample is collected, which is then smeared on a slide. The smear is stained with auramine- rhodamine dye and examined under a fluorescent microscope for the presence of fluorescing bacilli.

If fluorescing bacilli are observed under the microscope, the test is positive, indicating a TB infection. A negative result means no bacilli were detected, but it does not rule out a TB infection, especially in the early stages.

While the test is generally accurate, false positives or negatives may occur due to various factors. For example, a false positive may result from the presence of non-tuberculous mycobacteria, while a false negative can occur if the bacilli count is too low.

TB is typically treated with a combination of antibiotics for a period of 6 to 9 months. It's essential to follow the treatment regimen closely to ensure complete eradication of the bacteria and prevent the development of drug-resistant TB.

Regular hand hygiene, wearing a mask when around people with active TB, and following a healthy lifestyle to strengthen your immune system can help reduce the risk of infection. Additionally, vaccination with the BCG vaccine can offer protection against TB.

Those with symptoms suggestive of TB, individuals who have been in close contact with a person diagnosed with TB, and people with weakened immune systems should consider this test.

A positive test result indicates the presence of tubercle bacilli, suggestive of TB. Your doctor will initiate appropriate treatment and may also recommend further testing to determine the extent of the disease and to guide therapy.

The test detects the presence of tubercle bacilli but does not provide information about their susceptibility to anti-TB drugs. Additional tests may be required to detect drug-resistant TB.

The test involves the collection of a sputum sample, which is generally safe with minimal risk. However, producing a sputum sample can sometimes induce coughing, which may cause discomfort.

No special preparation is needed for the Auramine Rhodamine Stain Test. Just follow your healthcare professional's instructions regarding the collection of the sputum sample.

This depends on your doctor's advice. If you're receiving treatment for TB, your doctor may recommend periodic testing to monitor the effectiveness of the treatment.

This test is often used in combination with a chest X-ray and other laboratory tests like culture and molecular tests to confirm a diagnosis of TB.

You should consult a pulmonologist or an infectious disease specialist for further evaluation and treatment.

Tuberculosis continues to be a significant global health issue. Rapid detection and appropriate treatment are crucial to control its spread and impact. The Auramine Rhodamine Stain Test for Tubercle Bacilli represents a critical diagnostic tool in this battle, offering quick, efficient, and accurate detection of TB-causing bacteria. Armed with the right information and guidance, you can take proactive steps towards safeguarding your health against this formidable foe.

Auramine Rhodamine Stain for screening of Tubercle bacilli
₹ 500
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Locations Near You in Hyderabad
  • 4KM from Madhapur
  • 3KM from Banjara Hills
  • 1.9KM from Yusufguda
  • 3KM from Madhura Nagar
  • 5KM from Shaikpet